In vitro Regeneration of Aconitum balfourii Stapf: A Rare Medicinal Herb from Himalayan Alpine Through Root Explants
نویسندگان
چکیده
منابع مشابه
Optimization of DNA isolation protocol of Aconitum balfourii Stapf.: A rare medicinal herb of Himalayan alpine
Aconitum balfourii Stapf. (Family: Ranunculaceae) is a rare medicinal herb distributed at high altitude (2800-4200 m) of Himalayan region. It is rich in secondary metabolites, especially aconitines, having medicinal properties. Secondary metabolites, mainly polyphenols, cause hinderance in the isolation of good quality DNA from the plant. Therefore, in the present investigation, an attempt was ...
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In vitro multiple shoot regeneration of Solanum nigrum L., an Indian medicinal plant was accomplished on MS medium utilizing shoot tip and nodal explants. Direct multiple shoots differentiated within 6 weeks when explants were cultured on MS medium containing BAP (1.0-5.0 mg/l) and KIN (1.0-5.0 mg/l) individually. Among various concentrations of cytokinins tested, maximum number of multiple sho...
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Aconitm balfourii (Renunculaceae) is an important highly prized herb found in the Uttaranchal Himalaya in India. It has been frequently used in Ayurvedic systems of medicines since long time but unfortunately it is not taken up of for its systematic chemical investigation after 1928 when an alkaloid was reported to be present in its roots. In our program to search useful high altitude Himalayan...
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An efficient plant regeneration protocol was developed from leaf and internode (stem) explants of Bacopa monniera L. on MS basal medium, supplemented with Cytokinins like BAP, KN, and TDZ used singly. The leaf cultures expressed maximum plant regeneration potential with 138.9 ± 0.50 shoots per explants on MS + 2.0 mg/ l BAP. The in vitro regenerated dwarf shoots were further elongated on MS med...
متن کاملIn silico characterization and differential expression pattern analysis of conserved HMG CoA reductase domain isolated from Aconitum balfourii Stapf
The 3-hydroxy-3-methyl glutaryl CoA reductase (HMGR) is the key enzyme of mevalonate pathway in plants. A partial genomic DNA fragment encoding HMGR conserved domain (denoted as AbHMGR) is isolated from Aconitum balfourii Stapf. It comprises 871 bp encoding 290 amino acids. In silico analysis reveals that it had extensive similarities to other plant HMGR gene. Domain analysis of AbHMGR showed t...
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ژورنال
عنوان ژورنال: Research Journal of Medicinal Plant
سال: 2012
ISSN: 1819-3455
DOI: 10.3923/rjmp.2012.318.325